This is an outdated version published on 2024-07-02. Read the most recent version.

Application of Endophytic Bacteria from Tomato Stems to Control Bacterial Wilt Disease in Tomato and Enhance Plant Growth

Authors

  • Quang Trung Do Falculty of Biotechnology, Dai Nam University, Xom – Phu Lam – Ha Dong- Ha Noi - Vietnam
  • Danh Quang Do Falculty of Biotechnology, Dai Nam University, Xom – Phu Lam – Ha Dong- Ha Noi - Vietnam
  • Van Nha Pham Falculty of Biotechnology, Dai Nam University, Xom – Phu Lam – Ha Dong- Ha Noi - Vietnam
  • Thi Ngan Nguyen Falculty of Biotechnology, Dai Nam University, Xom – Phu Lam – Ha Dong- Ha Noi - Vietnam
  • Thi Thuy Linh Ngo Falculty of Biotechnology, Dai Nam University, Xom – Phu Lam – Ha Dong- Ha Noi - Vietnam
  • Thi Hong Van Pham Falculty of Biotechnology, Dai Nam University, Xom – Phu Lam – Ha Dong- Ha Noi - Vietnam
  • Trong Tri Nguyen Vietnam National University of Forestry, Xuan Mai - Ha Noi - Vietnam
  • Mai Van Dinh Hanoi University of Science, Vietnam National University Hanoi, 334 Nguyen Trai – Thanh Xuan - Ha Noi - Vietnam

Keywords:

Antagonistic ability, Bacterial wilt , Bacillus spp, endophytic bacteria, Ralstonia solanacearum

Abstract

Bacterial green wilt (BGW) disease, caused by Ralstonia solanacearum bacterium, is a devastating bacterial disease of tomatoes occurring in tropical zones, resulting in substantial yield losses in production fields. Biological control agents (BCAs), particularly plant endophytes such as bacteria, are becoming increasingly popular in microbial technology for cropping systems. In this study, we assessed the potential of endophytic bacteria from tomato plants to suppress the BGW caused by R. solanacearum in tomato plants. The research study revealed that out of the 9 endophytic strains isolated, Bacillus amyloliquefaciens TO3 demonstrated a strong antagonistic ability towards R. solanacearum, with the zone of inhibition approximately 16.58±0.19 mm. This strain also produced cell wall-degrading enzymes (amylase, protease, and cellulase), and indole acetic acid (IAA). In addition, the greenhouse experiments showed that applying TO3 before the infection of R. solanacearum led to a high protective effect against the BGW and a significant increase in plant height compared to the non-inoculated ones. In field conditions, inoculating tomato seedlings with strain TO3 resulted in a disease reduction of over 55% against the BGW compared to the non-treated control. Moreover, treatment with strain TO3 increased the yield of tomato fruits by up to 47.93% compared to non-treated control plants. These findings indicate the potential use of B. amyloliquefaciens TO3 in preventing the BGW disease caused by R. solanacearum on tomato plants.

References

Agarwal, H. et al. (2020). Endophytes from Gnetum gnemon L. can protect seedlings against the infection of phytopathogenic bacterium Ralstonia solanacearum as well as promote plant growth in tomato. Microbiological Research, 238, 126503. https://doi.org/10.1016/j.micres.2020.126503.

Amaresan, N. et al (2012). Endophytic bacteria from tomato and chili, their diversity and antagonistic potential against Ralstonia solanacearum. Archives of Phytopathology and Plant Protection, 45(3), 344-355. https://doi.org/10.1080/03235408.2011.587273

Achari, G.A. & Ramesh, R. (2018). Colonization of eggplant by endophytic bacteria antagonistic to Ralstonia solanacearum, the bacterial wilt pathogen. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 89, 585–593. https://doi.org/10.1007/s40011-018-0972-2

Bahmani, K. et al. (2021). Isolation and identification of endophytic bacteria from potato tissues and their effects as biological control agents against bacterial wilt. Physiological and Molecular Plant Pathology, 116, 101692. https://doi.org/10.1016/j.pmpp.2021.101692

Doan, T.T. & Nguyen, T.H. (2006). Status of research on biological control of tomato and groundnut bacterial wilt in Vietnam. Mitt Biol Bundesanst Land- Forstwirtsch, 408, 105-111.

Eid, A.M. et al. (2021). Harnessing bacterial endophytes for promotion of plant growth and biotechnological applications: An overview. Plants, 10(5), 935. https://doi.org/10.3390/plants10050935

Fahime, S. & Gholam, K. (2018). Endophytic bacteria suppress bacterial wilt of tomato caused by Ralstonia solanacearum and activate defence-related metabolites. Biological Journal of Microorganisms, 6(24), 39–52.

Fanhong, M. (2013). Ralstonia solanacearum species complex and bacterial wilt disease. Journal of Bacteriology and Parasitology, 4, 2. https://doi.org/10.4172/2155-9597.1000e119

Granér, G. et al. (2003). Screening of oxylipins for control of oilseed rape (Brassica napus) fungal pathogens. Phytochemistry, 63(1), 89-95. https://doi.org/10.1016/s0031-9422(02)00724-0.

Ji, X. et al. (2008). Biological control against bacterial wilt and colonization of mulberry by an endophytic Bacillus subtilis strain. FEMS Microbiology Ecology, 65(3), 565–573. https://doi.org/10.1111/j.1574-6941.2008.00543.x

Li, X. & de Boer, S.H. (1995). Selection of polymerase chain reaction primers from an RNA intergenic spacer region for specific detection of Clavibacter michiganensis subsp. spedonicus. Phytopathology, 85(8), 837-842.

Latha, P. et al. (2019). Endophytic bacteria: Prospects and applications for the plant disease management. In: Ansari R, Mahmood I (eds) Plant health under biotic stress. Springer, Singapore.

Mohamed, A.F. et al. (2020). Biological control of bacterial wilt of tomato caused by Ralstonia solanacearum using Pseudomonas species isolated from the rhizosphere of tomato plants. Archives of Phytopathology and Plant Protection, 53(1-2), 1-16. https://doi.org/10.1080/03235408.2020.1715756

Nguyen, T.H.H. et al. (2006). Bacterial endophyte: biological charateristics and application potential in biological control of bacterial wilt caused by Ralstonia solanacearum. Science and Technology Journal of Agriculture and Rural Development, 18(2), 77-79 (in Vietnamese).

Puente, M.E. et al. (2004). Microbial populations and activities in the rhizoplane of rock-weathering desert plants. I. Root colonization and weathering of igneous rocks. Plant Biology, 6, 629-642. https://doi.org/10.1055/s-2004-821100

Seleim, M. et al. (2011). Biological control of bacterial wilt of tomato by plant growth promoting rhizobacteria. Journal of Plant Pathology, 10, 146-153. https://doi.org/10.3923/ppj.2011.146.153

Singh, D. et al. (2022). Suppression of tomato bacterial wilt incited by Ralstonia pseudosolanacearum using polyketide antibiotic-producing Bacillus spp. isolated from rhizospheric soil. Agriculture, 12, 2009. https://doi.org/10.3390/agriculture12122009

Trung, D.Q. et al. (2023). Application of endophytic bacterium Bacillus velezensis BTR11 to control bacterial leaf blight disease and promote rice growth. Egyptian Journal of Biological Pest Control, 33, 97. https://doi.org/10.1186/s41938-023-00740-w

Vessey, J.K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, 255, 571–586. https://doi.org/10.1023/A:1026037216893

William, S. (1983). Staining reaction for detection of hemicellulose degrading. FEMS Microbiology Letters, 20, 253-258.

Downloads

Published

2024-07-02

Versions

Issue

Section

Plant Science