Application of Endophytic Bacteria from Tomato Stems to Control Bacterial Wilt Disease in Tomato and Enhance Plant Growth

Authors

  • Quang Trung Do Faculty of Biotechnology, Dai Nam University, Xom – Phu Lam – Ha Dong- Ha Noi - Vietnam
  • Danh Quang Do Faculty of Biotechnology, Dai Nam University, Xom – Phu Lam – Ha Dong- Ha Noi - Vietnam
  • Van Nha Pham Faculty of Biotechnology, Dai Nam University, Xom – Phu Lam – Ha Dong- Ha Noi - Vietnam
  • Thi Ngan Nguyen Faculty of Biotechnology, Dai Nam University, Xom – Phu Lam – Ha Dong- Ha Noi - Vietnam
  • Thi Thuy Linh Ngo Faculty of Biotechnology, Dai Nam University, Xom – Phu Lam – Ha Dong- Ha Noi - Vietnam
  • Thi Hong Van Pham Faculty of Biotechnology, Dai Nam University, Xom – Phu Lam – Ha Dong- Ha Noi - Vietnam
  • Trong Tri Nguyen Vietnam National University of Forestry, Xuan Mai - Ha Noi - Vietnam
  • Mai Van Dinh Hanoi University of Science, Vietnam National University Hanoi, 334 Nguyen Trai – Thanh Xuan - Ha Noi - Vietnam

Keywords:

Antagonistic ability, Bacterial wilt , Bacillus spp, endophytic bacteria, Ralstonia solanacearum

Abstract

Bacterial green wilt (BGW) disease, caused by Ralstonia solanacearum bacterium, is a devastating bacterial disease of tomatoes 
occurring in tropical zones, resulting in substantial yield losses in production fields. Biological control agents (BCAs), particularly 
plant endophytes such as bacteria, are becoming increasingly popular in microbial technology for cropping systems. In this study, 
we assessed the potential of endophytic bacteria from tomato plants to suppress the BGW caused by R. solanacearum in tomato 
plants. The research study revealed that out of the 9 endophytic strains isolated, Bacillus amyloliquefaciens TO3 demonstrated 
a strong antagonistic ability towards R. solanacearum, with the zone of inhibition approximately 16.58 ±0.19 mm. This strain 
also produced cell wall-degrading enzymes (amylase, protease, and cellulase), and indole acetic acid (IAA). In addition, 
the greenhouse experiments showed that applying strain TO3 before the infection of R. solanacearum led to a high protective 
effect against the  BGW and a  significant increase in plant height compared to the  non-inoculated ones. In field conditions, 
inoculating tomato seedlings with strain TO3 resulted in a disease reduction of over 55% against the BGW compared to the nontreated control. Moreover, treatment with strain TO3 increased the yield of tomato fruits by up to 47.93% compared to nontreated control plants. These findings indicate the potential use of B. amyloliquefaciens TO3 in preventing the BGW disease caused by R. solanacearum on tomato plants.

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Published

2024-07-02 — Updated on 2024-07-08

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Issue

Vol. 27 No. 2 (2024)

Section

Plant Science

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Copyright (c) 2024 Quang Trung Do, Danh Quang Do, Van Nha Pham, Thi Ngan Nguyen, Thi Thuy Linh Ngo, Thi Hong Van Pham, Trong Tri Nguyen, Mai Van Dinh

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