Study of polymorphism of the MC4R locus in pigs of the large white breed
Keywords:
genetic similarity, allele, genotype, pigs, geneAbstract
DNA typing was performed by the PCR-RFLP method at the loci of the MC4R gene The herd of pigs was divided according to origin. Polymorphism of the studied gene is established. The higher frequency of AA and AG genotypes was in animals of French breeding, and the homozygous GG genotype was in crossbred animals. Accordingly, allele A had a higher frequency in French breeding animals and allele B - in crossbred animals. According to the studied gene, genetic balance is absent only in crossbred animals. A high coefficient of genetic similarity was established between French and Belgian breeding animals. Most of the existing breeders have a heterozygous genotype.
References
Barb, C.R, et al. (2004). The role of melanocortin-3 and -4 receptor in regulating appetite, energy homeostasis and neuroendocrine function in the pig. Journal of Endocrinology, 181, 39-52.
Benoit, S. (2000). CNS Melanocortin System Involvement in the Regulation of Food Intake. Hormones and Behavior, 37(4), 299-305. https://doi.org/10.1006/hbeh.2000.1588
Budakva, E. O. et al. (2021). Determining the presence of polymorphisms of MC4R and IGF-2 productivity markers among non-castrated and immunologically castrated pigs of the final Irish hybrid (wb x l) x maxgro. International scientific journal "Grail of Science". 9, 151-155. https://doi.org/10.36074/grail-of-science.22.10.2021.23
Chen, M. et al. (2004). Different allele frequencies of MC4R gene variants in Chinese pig Breeds. Archiv fur Tierzucht., 47(5), 463-468. https://doi.org/10.5194/aab-47-463-2004
Dvořakova, V. et al. (2011). Effect of the missense mutation Asp298Asn in MC4R on growth and fatness traits in commercial pig crosses in the Czech Republic. Czech Journal of Animal Science, 56(4), 176–180. https://doi.org/10.17221/1305-CJAS
Glazko, V. I. (2001). DNA technologies and bioinformatics in solving problems of mammalian biotechnology: monograph. Publishing house BSAU, 2001.
Houston, R. D., et al. (2004). A melanocortin- 4 receptor (MC4R) polymorphism is associated with performance traits in divergently selected large white pig populations. Animal Genetics, 35, 386– 390. https://doi.org/10.1111/j.1365-2052.2004.01182.x
Kennes, Y. M. et al. (2001). Characterization of swine leptin (LEP) polymorphisms and their association with production traits. Animal Genetics, 32, 215-218. 10.1046/j.1365-2052.2001.00768.x
Kim, K. S. et al. (2000). Rapid communication: linkage and physical mapping of the porcine melanocortin-4 receptor (MC4R) gene. Journal of Animal Science, 78, 791– 792. https://doi.org/10.2527/2000.783791x
Kim, K. S. et al. (2004). Functional and phylogenetic analyses of a melanocortin- 4 receptor mutation in domestic pigs. Domestic Animal Endocrinology, 26, 75– 86. https://doi.org/10.1016/j.domaniend.2003.12.001
Kim, K. S. et al. (2006). Association of melanocortin 4 receptor (MC4R) and high mobility group AT-hook 1 (HMGA1) polymorphisms with pig growth and fat deposition traits. Animal Genetics, 37, 419–421. https://doi.org/10.1111/j.1365-2052.2006.01482.x
Li, C. L. et al. (2006). Polymorphism of the H-FABP, MC4R and ADD1 genes in the Meishan and four other pig population in China. South African Journal of Animal Science. 36(1), 1–6. https://scielo.org.za/pdf/sajas/v36n1/01.pdf
Likhach, V. Ya. et al. (2021). Genetic structure of pig populations of different breeds according to the CTSL AND MC4R genes. 118, 253-260 https://doi.org/10.32851/2226-0099.2021.118.32
Malikova, A. et al. (2024). Determination of Pit-1 and T Polymorphism in Ukrainian Brown Dairy and Sumy Intrabreed Type of the Ukrainian Black-and-White Dairy Cattle. Acta fytotechn zootechn, 27(3), 203-208. https://doi.org/10.15414/afz.2024.27.03.203-208
Malikova, A. et al. (2024).The genotypic profile of milk proteins in cattle populations created on the mother base of the Lebedinian breed. Acta fytotechn zootechn, 27(3), 187-192. https://doi.org/10.15414/afz.2024.27.03.187-192
Merkuryeva, E.K. (1977). Genetic basis of selection in cattle breeding. Ear
Muñoz G, et al. (2011).Effects of porcine MC4R and LEPR polymorphisms, gender and Duroc sire line on economic traits in Duroc × Iberian crossbred pigs. Meat Sci. 88(1),169−73. https://doi.org/10.1016/j.meatsci.2010.12.018
Pang, W. J. et al. (2006). Relationship among H-FABP gene polymorphism, intramuscular fat content, and adipocyte lipid droplet content in main pig breeds with different genotypes in western China. Acta Genetica Sinica, 33 (6), 515-24, 2006. https://doi.org/10.1016/S0379-4172(06)60080-2
Salajpal, K et al. (2007). Effect of MC4R polymorphism on physiological stress response in pigs Agriculture. Scientific and Professional Review, 13(1), 46 – 50.
Short, H.T. & Rothschild, F. M. (1997). Effect of the Estrogen Receptor Locus on Reproduction and Production Traits in Four Commercial Pig Lines. Animal Science Journal, 75, 3138-3142. https://doi.org/10.2527/1997.75123138x
Szyndler-Nкdza, M., et al. (2010). Effect of mutation in MC4R gene on carcass quality in Pulawska pig included in conservation breeding programmer. Animal Science Papers and Reports, 28(1), 37–45.
Walsh, P.S. et al. (1991). Chelex 100 as a Medium for Extraction of DNA for PCR-Based Typing from Forensic Material. BioTechniques, 10, 506-509.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Yuriy Skliarenko, Victor Khalak, Oleksandr Bordun, Bogdan Gutyj, Lyudmyla Zasukha, Anna Horchanok, Oksana Kuzmenko, Natalia Prisjazhnjuk
This work is licensed under a Creative Commons Attribution 4.0 International License.
