Variabily of Amaranthus Hypochondriacus L. x Amaranthus Hybridus L. var. Plainsman After Cd and Si Treatment Detected by CDDP and PBA Marker Techniques
Keywords:
CDDP, PBA, heavy metals, phytoremediation, abiotic stressAbstract
During evolution, plants have developed complex mechanisms to cope with biotic and abiotic stresses. The study of heavy metal-plant interactions, particularly at the molecular genetic level, helps to understand heavy metal accumulation in plants and their resistance to heavy metal-induced stress. Amaranthus spp. have a potential in phytoremediation techniques. In this study was analysed the effects of cadmium (Cd) treatment and Cd treatment with addition of silicon (Si) on Amaranthus hypochondriacus L. x Amaranthus hybridus L. var. Plainsman, in leaves, in the context of the molecular genomic response using two different DNA marker techniques such as cytochrome P450 mono-oxygenase analogues (PBA) and conserved DNA-derived polymorphism (CDDP) for WRKY genes. The PBA marker technique consists of several primers pairs that have been designed for cytochrome P450 genes, and CDDP primers pairs have been designed on conserved regions of DNA. Both marker techniques have been shown to be able to detect the response to biotic and abiotic stresses. We detected higher variability in genomic profile with the CDDP marker systems compared to PBA. However, the highest number of loci (169) we detected using the BF (forward primer) + BR (reverse primer) of PBA.
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