Diversity and fungicidal resistance of Cercospora beticola
Keywords:Cercospora beticola, sporulation, fungicides, resistance
The fungus Cercospora beticola Sacc. belongs to the most important pathogens on sugar beet. The Cercospora leaf spot disease is important problem for growers in all growing areas of sugar beet. This study was focused on the morphological diversity of C. beticola isolates and occurrence of their fungicidal resistance in Slovakia. Isolates involved in this work were collected from sugar beet leaf during 2016-2018. Average growth rate of the tested C. beticola isolates on five different media showed the major role of cultivation temperature. The fastest growth was measured by 30°C on TE medium, followed by PDA, SBLEA, AWSBL, and V8. The colour of aerial mycelium varied from olive-green to grey with white powdery appearance, per grey colour with wrinkled texture, to black colour. There is no significant correlation among isolates origin, colony morphology parameters and growth rate. The highest sporulation rate was recorded at cultivation temperature 25°C by using of TE growth medium, the lowest one by V8 medium. The inhibition effect of azoxystrobin + cyproconazole was significantly different among the tested isolates from different localities and years. The results showed reduced sensitivity of C. beticola population in Slovakia strictly depending of the locality. The most reduced sensitivity was measured on localities Hronovce and Nové Zámky, followed by localities Mojmírovce, Senec, and Dolné Saliby with similar values. There is no positive correlation between inhibition effect and mycelial growth rate. The results showed increasing fungicidal resistance of C. beticola to azoxystrobin + cyproconazole in some localities of Slovakia.
Balandžić, M. et al. (2020). Sensitivity of cercospora beticola isolates to azoxystrobin. Contemporary Agriculture, 69(1-2), 1-4. https://doi.org/10.2478/contagri-2020-0001
Bolton, M. D. et al. (2012). Characterization of CbCyp51 from field isolates of Cercospora beticola. Phytopathology, 102(3), 298-305. https://doi.org/10.1094/PHYTO-07-11-0212
Budakov, D. et al. (2014). Sensitivity of Cercospora beticola isolates from Serbia to carbendazimand and flutriafol. Crop Protection, 66(12), 120–126. https://doi.org/10.1016/j.cropro.2014.09.010
Esh, A.M.H. & Moghaieb, R.E.A. (2011). Analysis of morphological, pathological and genotypic diversity in Cercospora beticola sacc. from different sugar beet cultivation in Egypt. Arab Journal of Biotechnology, 14(1), 77-88. https://www.researchgate.net/publication/259975630
Groenewald, M. et al. (2005). Distinct species exist within the Cercospora apii morphotype. Phytopathology, 95(8), 951-959. https://doi.org/10.1094/PHYTO-95-0951
Groenewald, M. et al. (2008). Indirect evidence for sexual reproduction in Cercospora beticola populations from sugar beet. Plant Pathology, 57(1), 25–32. https://doi.org/10.1111/j.1365-3059.2007.01697.x
Karadimos, D.A. & Karaoglanidis, G.S. (2006). Comparative efficacy, selection of effective partners and application time of strobilurin fungicides for control of Cercospora leaf-spot of sugar beet. Plant Disease, 90 (6), 820–825. https://doi.org/10.1094/PD-90-0820
Karaoglanidis, G.S. & Thanassoulopoulos, C.C. (2003). Cross-resistance patterns among sterol biosynthesis inhibiting fungicides (SBIs) in Cercospora beticola. European Journal of Plant Pathology, 109(9), 929–934.https://doi.org/10.1023/B:EJPP.0000003672.36076.8a
Karaoglanidis, G. S. et al. (2002). Changes in sensitivity of Cercospora beticola populations to sterol-demethylation-inhibiting fungicides during a 4-year period in northern Greece. Plant Pathology, 51(1), 55–62. https://doi.org/10.1046/j.0032-0862.2001.x-i2
Karaoglanidis, G. S. et al. (2003). Sensitivity of Cercospora beticola populations to fentin-acetate, benomyl and flutriafol in Greece. Crop Protection, 22(5), 735–740. https://doi.org/10.1016/S0261-2194(03)00036-X
Ma, Z. & Michailides, T.J. (2005). Advances in understanding molecular mechanisms of fungicide resistance and molecular detection of resistant genotypes in phytopathogenic fungi. Crop Protection, 24(10), 853–863. https://doi.org/10.1016/j.cropro.2005.01.011
Malandrakis, A.A. et al. (2006). Biological and molecular characterization of laboratory mutants of Cercospora beticola resistant to Qo inhibitors. European Journal of Plant Pathology, 116(2), 155–166. https://doi.org/10.1007/s10658-006-9052-1
Moretti, M. et al. (2006). Analysis of genotypic diversity in Cercospora beticola Sacc. field isolates. Annals of Microbiology, 56(3), 215-221. https://doi.org/10.1007/BF03175008
Nikou, D. et al. (2009). Molecular characterization and detection of overexpressed C-14 alpha-demethylase-based DMI resistance in Cercospora beticola field isolates. Pesticide Biochemistry and Physiology, 95(1), 18–27. https://doi.org/10.1016/j.pestbp.2009.04.014
Piszczek, J. et al. (2018). First report of G143A strobilurin resistance in Cercospora beticola in sugar beet (Beta vulgaris) in Poland. Journal of Plant Diseases Protection, 125(1), 99–101. https://doi.org/10.1007/s41348-017-0119-3
Shane, W.W. & Teng, P.S. (1992). Impact of Cercospora leaf spot on root weight, sugar yield, and purity of Beta vulgaris. Plant Diseases. 76(1), 812-820. https://doi.org/10.1094/PD-76-0812.
Silva, M.G.D. et al. (2016). Effect of light and temperature on Cercospora coffeicola and Coffea arabica pathosystem. Coffe Science, 11(2), 148-160. http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/1012.
Skaracis, G.N. et al. (2010). Cercospora leaf spot disease of sugar beet. Sugar Technology, 12 (3–4), 220–228. https://doi.org/10.1007/s12355-010-0055-z
Tumbek, A.C. et al. (2011). Sensitivity of Cercospora beticola populations in Turkey to flutriafol, mancozeb, and fentin acetate. Turkish Journal of Agriculture and Forestry, 35(1), 65–71. https://doi.org/10.3906/tar-0910-24
Upchurch, R. G. et al. (1991). Mutants of Cercospora kikuchii altered in cercosporin synthesis and pathogenicity. Appied Environmental Microbiology, 57(10), 2940-2945. https://doi.org/10.1128/aem.57.10.2940-2945.1991
Uppala, S.S. et al. (2019). Plant-Based Culture Media for Improved Growth and Sporulation of Cercospora janseana. Plant Disease, 103(3), 504-508. https://doi.org/10.1094/PDIS-05-18-0814-RE
Copyright (c) 2022 Acta Fytotechnica et zootechnica
This work is licensed under a Creative Commons Attribution 4.0 International License.