Effects of Sage (Salvia officinalis L.) Supplementation in Hen Diets on Laying Productivity, Egg Quality, and Biochemical Parameters


  • Maria Todorova Agricultural Academy, Sofia, Institute of Animal Science, sp. Pochivka, 2232 Kostinbrod, Bulgaria
  • Svetlana Grigorova Agricultural Academy, Sofia, Institute of Animal Science, sp. Pochivka, 2232 Kostinbrod, Bulgaria
  • Natasha Gjorgovska University Ss. Ciril and Methodius in Skopje, Institute of Animal Science and Fishery


Salvia officinalis L., hens, egg morphological properties, egg chemical composition, sensory indicators


An experiment was designed to examine the effect of sage leaves (Salvia officinalis. L.) use in hens’ diet on laying productivity, egg morphological and sensor properties, serum biochemistry, and egg chemical composition. A total of 90 hens from the Bulgarian synthetic population LB at the age of 40 weeks were randomly divided into three groups: S0 (without dried and milled leaves of  S. officinalis L supplementation) and S25, and S50 experimental groups (diets supplemented with dried and milled leaves of S. officinalis L. at 0.25% and 0.50% respectively). The sage use in the daily dose of 0.50% led to a significant increase in hens’ body weight (P <0.05); laying intensity (P <0.05); Haugh Units (P <0.01); albumen index (P <0.01) and egg yolk color (P <0.001). The addition of 0.25% S. officinalis significantly decreased the total cholesterol level in the blood serum (P <0.05) but significantly increased the content of protein (P <0.001); fat (P <0.05), and ash (P <0.01; P <0.05) in eggs. The supplementation of the tested product does not aggravate the eggs’ sensor properties.

Author Biographies

  • Maria Todorova, Agricultural Academy, Sofia, Institute of Animal Science, sp. Pochivka, 2232 Kostinbrod, Bulgaria

    Department Animal Nutrition and Feed Management


  • Svetlana Grigorova, Agricultural Academy, Sofia, Institute of Animal Science, sp. Pochivka, 2232 Kostinbrod, Bulgaria

    Department of Nutrition and Feed Management

    Assoc. professor


AOAC. (2007). Official Methods of Analysis. (18th ed.), Association of Official Analytical chemists, Gaithersburg.

Bulgarian State Standards 4336-73. (1990). Eggs and egg products. Rules for taking samples for research. Food Industry. Poultry and their products. Standardization. Sofia, Bulgaria. 5 Part IV (pp. 117–180).

Chen H., Li D., Chang B., Gong L., Dai J. and Yi G. (2003). Effects of Chinese herbal polysaccharides on the immunity and growth performance of young broilers. Poultry Science, 82, 364–370. DOI: https://doi.org/10.1093/ps/82.3.364

Deeseenthum, S., & J. Pejovic (2010). Bacterial inhibition and antioxidant activity of kefir produced from Thai Jasmine rice milk. Biotechnology, 9(3), 332–337. DOI: https://doi.org/10.3923/biotech.2010.332.337

Draz S.N., Ali A.M.F., Hussein K.E., & Afifi A.K.S. (2021). A comparative study between green tea (Camellia sinensis) and common sage (Salvia officinalis) (Chemical composition and active(compounds). Menoufia Journal of Agricultural Biotechnology, 6(1), 11–21. DOI: https://doi.org/10.21608/mjab.2021.155478

El-Garhy O.H. (2018). Effect of dietary sage plant leaves powder and ascorbic acid supplementation on the performance of broiler chickens under Iraq conditions. Annals of Agricultural Science Moshtohor, 56(4), 37–46. DOI: https://doi.org/10.21608/assjm.2018.57306

El-Ghany, W.A.A. (2023). A natural feed additive phytobiotic, pomegranate (Punica granatum L.), and the health status of poultry. Macedonian Veterinary Review, 46(2), 113–128. DOI: https://doi.org/10.2478_macvetrev_2023_0022

El-Rahman Abor M., & Abd M. (2018). Therapeutic effect of leafy herbs (Camellia sinensis, Malva parviflora, Rosmarinus officinalis and Salvia officinalis) as a tool for anti-overobesity. Home Economics Journal, 34, 42–60. DOI: https://doi.org/10.21608/jhe.2018.79829

Farhadi M., Hedayati M., Manafi M., & Khalaji S. (2020). Influence of using sage powder (Salvia officinalis) on performance, blood cells, immunity titers, biochemical parameters and small intestine morphology in broiler chickens. Iranian Journal of Applied Animal Science, 10(3), 509–516. DOI: https://doi.org/20.1001.1.2251628.2020.

Fasseas M.K., Mountzairis C. K., Tarantilis A.P., Polissiou M., & Zervas G. (2008). Antioxidant activity in meat, treated with oregano and sage essential oils. Food Chemistry, 106(3),1188–1194. DOI: https://doi.org/10.1016/j.foodchem.2007.07.060

Frankic T., Volic M., Salobir J., & Rezar V. (2009). Use of herbs and spices and their extract in animal nutrition. Acta Agriculturae Slovenica, 94(2), 95–102. DOI: https://doi.org/10.14720/aas.2009.94.2.14834

Galamatis D., Papadopoulos G.A., Lazari D., Fletouris D., Petridou E., Arsenos G. L., & Fortomaris P.I. (2021). Effects of dietary supplementation of Salvia officinalis l. in organic laying hens on egg quality, yolk oxidative stability and eggshell microbiological counts. Animals, 11(9), 2502. DOI: https://doi.org/10.3390/ani11092502

Grigorova S. (2014). Sources and importance of natural antioxidants in animal nutrition. Zhivotnovdni nauki, 51, 46–52 (Bg).

Hernandez, F., Madrid J., Garcia V., Orengo J., & Megias M.D. (2004). Influence of two plant extracts on broilers performance digestibility and digestive organ size. Poultry Science, 83(2), 169–174. DOI: https://doi.org/10.1093/ps/83.2.169

Kara K., Güçlü B.K., Baytok E., & Şentürk M. (2016). Effects of grape pomace supplementation to laying hen diet on performance, egg quality, egg lipid peroxidation and some biochemical parameters. Journal of Applied Animal Research, 44(1), 303–310. https://doi.org/10.1080/09712119.2015.1031785

Khalil E., Esoh R., Rababah T., Almajwal A., & Alu‘datt M.H. (2012). Minerals, proximate composition and their correlations of medicinal plants from Jordan. Journal of Medicinal Plants Research, 6(47), 5757–5762. DOI: https://doi.org/10.5897/JMPR10.022

Landjev I. (2010). Encyclopedia of medicinal plants in Bulgaria (Ed. Deleva N.) (p. 551). Trud Book Publishing House (Bulgaria).

Levkut M., Marcin A., Lenhardt L., Porvaz P., Revajová V., Šoltysová B., Blanár J., Ševčíková Z., & Pist J. (2010). Effect of sage extract on alkaline phosphatase, enterocyte proliferative activity and growth performance in chickens. Acta Veterinaria Brno, 79(2), 177–183. DOI: https://doi.org/10.2754/avb201079020177

Mollova S., Damyanova St., Kostova I., Stoilova I., & Stoyanova A. (2016). Chemical composition, antioxidant and antimicrobial activities of extracts from sage (Salvia officinalis L.). Proceedings of Univeresity of Ruse, 55, 75–82 (Bulgaria).

Neagu E., Paun G., & Radu G.L. (2014). Chemical composition and antioxidant activity of Salvia officinalis concentrated by ultrafiltracion. Romanian Biotechnological Letters, 19(2), 9203–9211. https://rombio.unibuc.ro/wp-content/uploads/2022/05/19-2-13.pdf

Ommati M.M., Zamiri M.J., Akhlaghi A., Atashi H., Jafarzadeh M.R., Rezvani M.R., & Saemi F. (2013). Seminal characteristics, sperm fatty acids, and blood biochemical attributes in breeder roosters orally administered with sage (Salvia officinalis) extract. Animal Production Science, 53(6), 548–554. DOI: https://doi.org/10.1071/AN12257

Piesova E., Makova Z., Levkut M., Faixova Z., Pistl J., Marcin A., & Levkut M. (2012). The effects of sage extract feed supplementation on biochemical parameters, weight of internal organs and Salmonella counts in chickens. Research in Veterinary Science, 93(3), 1307–1308. DOI: https://doi.org/10.1016/j.rvsc.2012.04.011

Rasouli B., Movahhedkhah S., Seidavi A., Haq Q.M.I., Kadim I., Laudadio V., Mazzei D., & Tufarelli V. (2020). Effect of sage (Salvia officinalis L.) aqueous leaf extract on performance, blood constituents, immunity response and ileal microflora of broiler chickens. Agroforestry Systems, 94(4), 1179–1187. DOI: https://doi.org/10.1007/s10457-019-00401-8

Saleh A.A., Hamed S., Hassan A.M., Amber K., Awad W., Alzawqari M.H., & Shukry M. (2021). Productive performance, ovarian follicular development, lipid peroxidation, antioxidative status, and egg quality in laying hens fed diets supplemented with Salvia officinalis and Origanum majorana powder levels. Animals, 11(12), 3513. https://doi.org/doi.org/10.3390/ani11123513

Szabóová, R., Lauková A., Chrastinová L., Simonová M., Strompfová V., M. Haviarová M., Plachá I., Faix Š., Vasilková Z., Chrenková M., & Rafay J. (2008). Experimental application of sage in rabbit husbandry. Acta Veterinaria, 77(4), 581–588. DOI: https://doi.org/10.2754/avb200877040581

Todorova M. (2022) Mentha spicata and Salvia officinalis L. in compound feed for poultry. Zhivotnovodni Nauki, 59(6), 63–72 (Bg).

Todorov N., Marinov B., Ilchev A., Kirilov A., S. Chobanova S., & Ganchev G. (2021). Basics of animal nutrition. (p. 464) Book Publishing House, St. Zagora (Bulgaria).

Traesel C.K., Wolkmer P., Schmidt C., Silva C.B., Paim F.C., Rosa A. P., & Lopes S.T. (2011). Serum biochemical profile and performance of broiler chickens fed diets containing essential oils and pepper. Comparative Clinical Pathology, 20(5), 453–460. DOI: https://doi.org/10.1007/s00580-010-1018-1






Animal Science