Phosphate-solubilizing bacteria enhance the growth and lead removal of weed plants (Echinochloa colona)
Keywords:
Pseudomonas putida, phytoremediation, soil fertility, metal-contaminated soil, weedAbstract
Heavy metal pollution of soils in being a serious problem for sustainable agriculture. A promising solution for phytoremediation of metal-contaminated soils is to use plants in combination with phosphate-solubilizing bacteria (PSB). In this study, a total of 30 soil samples were collected from different locations in Nam Dinh, Vietnam. They were used to isolate PSB from paddy soil on Pikovskaya agar media, and their ability in improving the phytoremediation of lead (Pb2+) by a weed plant (Echinochloa colona) as well as in promoting the growth of E. colona under Pb stress condition was investigated by pot experiments. Total 07 PSB were isolated and the ND04 showed the ability in solubilizing multiple P sources ( Ca3(PO4)2, AlPO4, FePO4, and phytate) with corresponding P solubilizing levels were 530.12, 50.13, 25.02, and 3.58 mg/L PO43–-P, respectively. Moreover, the ND04 strain was identified as Pseudomonas putida (accession number FJ976605.1) and produced the highest values of available P (1.67 mg/L) in Ca3(PO4)2 ‑incubated soil experiments. Furthermore, the ND04 inoculation significantly enhanced the growth of E. colona and also increased the phytoremediation efficiency of Pb from Pb-contaminated soil. These results suggest the ND04 could potentially use to construct novel constructed wetlands for phytoremediation of metal-contaminated soil.
References
Aransiola, S.A. et al. (2019). Microbial-aided phytoremediation of heavy metals contaminated soil: a review. European Journal of Biological Research, 9(2), 104-125. https://doi.org/10.5281/zenodo.3244176.
Aliyat, F.Z. et al. (2022). Phosphate-solubilizing bacteria isolated from phosphate solid sludge and their ability to solubilize three inorganic phosphate forms: Calcium, Iron, and Aluminum Phosphates. Microorganisms, 10, 980. https://doi.org/10.3390/ microorganisms10050980
Adhikari, A. et al. (2020). Effect of Silicate and Phosphate Solubilizing Rhizobacterium Enterobacter ludwigii GAK2 on Oryza sativa L. under Cadmium Stress. Journal of Microbiology and Biotechnology, 30(1), 118-126. https://doi.org/10.4014/jmb.1906.06010.
Bortoloti, G. & Baron, D. (2022). Phytoremediation of toxic heavy metals by Brassica plants: a biochemical and physiological approach. Environmental Advances, 8, 100204. https://doi.org/10.1016/j.envadv.2022.100204.
Fankem, H. et al. (2006). Occurrence and Functioning of Phosphate Solubilizing Microorganisms from Oil Palm Tree (Elaeis guineensis) Rhizosphere in Cameroon. African Journal of Biotechnology, 5(24), 2450-2460.
Himani, S. & Reddy, M.S. (2011). Effect of inoculation with phosphate solubilizing fungus on growth and nutrient uptake of wheat and maize plants fertilized with rock phosphate in alkaline soils. European Journal of Soil Biology, 47(1), 30–34. https://doi.org/10.1016/j.ejsobi.2010.10.005.
Jones, J.B. & Case, V.W. (1990). Sampling, handling, and analyzing plant tissue samples. In Westerman RL (ed.). Soil Testing and Plant Analysis. Soil Science Society of America, Inc., Madison, WI, pp. 389-447.
Kumar, A. & Rai, L.C. (2015). Proteomic and biochemical basis for enhanced growth yield of Enterobacter sp. LCR1 on insoluble phosphate medium. Microbiology Research, 170, 195-204. https://doi.org/10.1016/j.micres.2014.06.006.
Katiyar, V. & Goel, R. (2003). Solubilization of inorganic phosphate and plant growth promotion by cold tolerant mutants of Pseudomonas fluorescens. Microbiology Research, 158(2), 163-168. https://doi.org/10.1078/0944-5013-00188.
Lai, W. et al. (2022). Combination of biochar and phosphorus solubilizing bacteria to improve the stable form of toxic metal minerals and microbial abundance in Lead/Cadmium-contaminated soil. Agronomy,12, 1003. https://doi.org/10.3390/ agronomy12051003.
Luu, T.A et al. (2021). Antagonistic activity of endophytic bacteria isolated from weed plant against stem end rot pathogen of pitaya in Vietnam. Egyptian Journal of pest biocontrol, 31(14), 1-8. https://doi.org/10.1186/s41938-021-00362-0.
Lin, M. et al. (2018). Phosphate-solubilizing bacteria improve the phytoremediation efficiency of Wedelia trilobata for Cu-contaminated soil. International Journal of Phytoremediation, 20(8), 813-822. https://doi.org/10.1080/15226514.2018.1438351.
Marra, L.M. et al. (2019). The amount of phosphate solubilization depends on the strain, C-source, organic acids and type of phosphate. Geomicrobiology Journal, 36(3), 232-242. https://doi.org/10.1080/01490451.2018.1542469.
Nakhro, N. & Dkhar, M.S. (2010). Impact of organic and inorganic fertilizers on microbial population and biomass carbon in paddy field soil. Journal of Agronomy, 9(3), 102-110. https://doi.org/10.3923/ja.2010.102.110.
Noble, A. et al. (2018). The Effect of Ripe Plantain Peels Waste on the Phytoextraction of Pb and Cd by Echinochloa colona (L.) Link. International Journal of Natural Resource Ecology and Management, 3(1),19. https://doi.org/10.11648/j.ijnrem.20180301.13.
Pikovskaya, R.I. (1948) ‘Mobilization of phosphorus in soil in connection with the vital activity of some microbial species’, Mikrobiologiya, 17, 362–370.
Pandey, A. et al. (2006). Characterization of a phosphate solubilizing and antagonistic strain of Pseudomonas putida (B0) isolated from a sub-Alpine location in the Indian Central Himalaya. Current Microbiology, 53(2), 102-7. https://doi.org/10.1007/s00284-006-4590-5.
Steadman, K. et al. (2004). Maturation temperature and rainfall influence seed dormancy characteristics of annual ryegrass (Lolium rigidum). Australian Journal of Agricultural Research, 55(10), 1047-1057. https://doi.org/10.1071/AR04083.
Stevenson, F.J. (2005). Cycles of Soil: Carbon, Nitrogen, Phosphorus, Sulfur, Micronutrients. John Wiley and Sons, Hoboken.
Subhashini, V. & Swamy, A.V.V.S. (2016). Efficiency of Echinochloa Colona in the removal of heavy metals from contaminated soils. International Journal of Scientific Research, 5(3), 689. https://doi.org/10.36106/ijsr.
Sánchez-Cruz, N.D. et al. (2020). Phosphate solubilization and indole-like compounds production by bacteria isolated from forest soil with plant growth promoting activity on pine seedlings. Geomicrobiology Journal, 37(10), 909-918. https://doi.org/10.1080/01490451.2020.1797945.
Tangahu, B.V. et al. (2011). Review on heavy metals (As, Pb, and Hg) uptake by plants through phytoremediation. International Journal of Chemical Engineering, 2011, 939161. https://doi.org/10.1155/2011/939161.
TCVN 4046 – 85 (1985). Agricultural soil – Sampling Method. State Committee for Science and Technology, Vietnam (in Vietnamese).
Teng, Z. et al. (2019). Characterization of phosphate solubilizing bacteria isolated from heavy metal contaminated soils and their potential for lead immobilization. Journal of Environmental Management, 231, 189-197. https://doi.org/10.1016/j.jenvman.2018.10.012.
Walpola, B.C. & Yoon, M.H. (2013). in vitro solubilisation of inorganic phosphates by phosphate solubilizing microorganisms. African Journal of Microbiology Research, 7, 3534-3541. https://doi.org/10.5897/AJMR2013.5861.
Wan, W. et al. (2020). Isolation and characterization of phosphorus solubilizing bacteria with multiple phosphorus sources utilizing capability and their potential for lead immobilization in soil’, Frontier Microbiology, 11, 752. https://doi.org/10.3389/fmicb.2020.00752.
Waterlot, C. (2018). Alternative approach to the standard, measurements and testing program used to establish phosphorus fractionation in soils. Analytic Climica Acta, 1003, 26–33. https://doi.org/10.1016/j.aca.2017.11.059.
Xiao, C. et al. (2021). Enhanced reduction of lead bioavailability in phosphate mining wasteland soil by a phosphate-solubilizing strain of Pseudomonas sp., LA, coupled with ryegrass (Lolium perenne L.) and sonchus (Sonchus oleraceus L.). Environmental Pollution, 274, 116572. https://doi.org/10.1016/j.envpol.2021.116572.
Yahaghi, Z. et al. (2018). Isolation and characterization of Pb-solubilizing bacteria and their effects on Pb uptake by Brassica juncea: Implications for microbe-assisted phytoremediation. Journal of Microbiology and Biotechnology, 28(7):1156-1167. https://doi.org/10.4014/jmb.1712.12038.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Trung Do, The Anh Luu, Minh Truong Dao
This work is licensed under a Creative Commons Attribution 4.0 International License.
