Optimization, characterization and biological effect of Algerian propolis
Keywords:
biological activities, experimental design, high liquid performance chromatography (HPLC), propolis, sperm motility.Abstract
Natural products was the source of many important biological activities against human and animal pathogens. Propolis was one of these substances, produced by bees from resins and wax. In this study, D-optimal experimental design used to optimize operational condition of propolis extraction, and then phenolic compounds of propolis extracted from seven regions of Bejaia was identified and quantified by HPLC analysis. Also, their biological activities was evaluated, including antibacterial, antifungal, antioxidant, anti-inflammatory effects, and sperm cells cryopreservation effect. The highest antibacterial activitywas obtained in propolis extract from the Baccaro region against B. cereus and strong effect against S. areus, C. albicans, and E. Coliwas reached using propolis extracted from Melbou region. High inhibition percentage denaturation of the BSA protein was observed using propolis extracted from Melbou. Also, the best antioxidant and sperm cells cryopreservation preservation was obtained using propolis extracted from Melbou. This work demonstrates that Melbou propolis extract obtained by the agitation method (MEA) showed the best and strongest biological effects, indicating an interesting and promising approach for development of new therapeutics formulations based on propolis extract from bejaia region.
References
Abdullah, N. A. et al. (2020). Phytochemicals, mineral contents, antioxidants, and antimicrobial activities of propolis produced by Brunei stingless bees Geniotrigona thoracica, Heterotrigona itama, and Tetrigona binghami. Saudi Journal of Biological Sciences, 27(11), 2902-2911.
Abdel Raheem, I. A. et al. (2019). Design, Evaluation And Antimicrobial Activity Of Egyptian Propolis-Loaded Nanoparticles : Intrinsic Role As A Novel And Naturally Based Root Canal Nanosealer. International Journal ofNanomedicine, 14, 8379-8398.
Ahmad, A., and Viljoen, A. (2015). The in vitro antimicrobial activity of Cymbopogon essential oil (lemon grass) and its interaction with silver ions. Phytomedicine, 22 (6), 657-665.
Ashry, E. S. H. E., and Ahmad, T. A. (2012). The use of propolis as vaccine’s adjuvant.Vaccine, 31(1), 31-39.
Bachevski, D. et al. (2019). Back to the basics : Propolis and COVID -19. Dermatologic Therapy,3(4).
Boufadi, Y. M. et al. (2016). Antimicrobial effects of six Algerian propolis extracts. International Journal ofFood Science Technology, 51(12), 2613‑2620.
Cao, J. et al. (2017). Ultrasound-assisted ionic liquid-based micellar extraction combined with microcrystalline cellulose as sorbent in dispersive microextraction for the determination of phenolic compounds in propolis. Analytica Chimica Acta, 963, 24-32.
Cui-ping, Z. et al. (2014). Development of High-Performance Liquid Chromatographic for Quality and Authenticity Control of Chinese Propolis : Quality control of propolis. Journal of Food Science, 79(7), C1315-C1322.
Demir, S. et al. (2021). Antiviral activity of different extracts of standardized propolis preparations against HSV. Antiviral Therapy, 25(7), 353-363.
Kashiwakura, J. et al. (2021). Propolis suppresses cytokine production in activated basophils and basophil-mediated skin and intestinal allergic inflammation in mice. Allergology International, 70(3), 360‑367.
Katalinic, V. et al. (2006). Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry, 94 (4), 550-557.
Kasote, D. M. et al. (2017). HPLC, NMR based chemical profiling and biological characterisation of Indian propolis,Fitoterapia, 122, 52-60.
Lekouaghet, A. et al. (2020). In vitro evaluation of antioxidant and anti-inflammatory activities of the hydroalcoholic extract and its fractions from Leuzea conifera L. roots. South African Journal of Botany,132, 103-107.
Lopes-Lutz, D. et al. (2008). Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry, 69(8), 1732-1738.
Marly, S. S. et al. (2018). Propolis as natural additive : A systematic review. African Journal Biotechnology, 17(41), 1282-1291.
Mortimer, S.T. (2000). CASA—Practical Aspects. Journal of Andrology, 21, 515–524.
Nedji, N., and Loucif-Ayad, W. (2014). Antimicrobial activity of Algerian propolis in foodborne pathogens and its quantitative chemical composition. Asian Pacific Journal of Tropical Disease, 4(6), 433-437.
Nina, N. et al. (2016). Chemical profiling and antioxidant activity of Bolivian propolis : Chemical profiling and antioxidants from Bolivian propolis. Journal of The Science of Food And Agriculture, 96(6), 2142-2153.
Niazian, M. (2019). Application of genetics and biotechnology for improving medicinal plants. Planta, 249 (4), 953-973.
Oroian, M. et al. (2020). Influence of ultrasonic amplitude, temperature, time and solvent concentration on bioactive compounds extraction from propolis.Ultrasonics Sonochemistry, 64, 105021.
Ö ğretmen, F. et al. (2014). Protective effects of propolis on cryopreservation of common carp (Cyprinuscarpio) sperm. Cryobiolgy, 68(1),107-112.
Pavlovic, R. et al. (2020). Effectiveness of Different Analytical Methods for the Characterization of Propolis : A Case of Study in Northern Italy. Molecules, 25(3), pp. 504.
Permana, A. D. et al. (2020). Phytosomal nanocarriers as platforms for improved delivery of natural antioxidant and photoprotective compounds in propolis : An approach for enhanced both dissolution behaviour in biorelevant media and skin retention profiles. Journal of Photochemistry And Photobiology B: Biology, 205, 111846.
Przybyłek, I., and Karpiński, T. M. (2019). Antibacterial Properties of Propolis. Molecules, 24(11), 2047.
Ramón-Sierra, J. et al. (2019). Partial characterization of ethanolic extract of Melipona beecheii propolis and in vitro evaluation of its antifungal activity. Revista Brasileira de Farmacognosia, 29(3), 319-324.
Suleman, T. et al. (2015). Antimicrobial activity and chemometric modelling of South African propolis.Journal of Applied Microbiology,119(4), 981-990.
Shahbaz, M. et al. (2021). Chemical profiling, HPLC characterization and in-vitro antioxidant potential of Pakistani propolis collected from peripheral region of Faisalabad. Cellular And Molecular Biology, 67(1), 40.
Sahlan, M. et al. (2019). Anti-inflammatory activity of Tetragronula species from Indonesia. SaudiJournal of Biological Sciences, 26(7), 1531‑1538.
Seven, I. et al.(2020). Bee glue (propolis) improves reproductive organs, sperm quality and histological changes and antioxidant parameters of testis tissues in rats exposed to excess copper. Andrologia, 52(4), 1-11.
Yusof, N. et al. (2021). Optimization of total phenolic compounds extracted from propolis by ultrasound- assisted extraction. Chemical Engineering Communications, 208(4), 564-572.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 ZAHRA TOUTOU, SOFIANE FATMI, Nacera CHIBANI, Leila BENSIDHOUM, Mohamed SKIBA, Mokrane IGUEROUADA
This work is licensed under a Creative Commons Attribution 4.0 International License.
